《大鼠补充磷虾油增强认知功能和抗抑郁作用》Karin Wibrand,Kjetil Berge et al. Lipids in Health and Disease 2013, 12:6|中冠生物科技(珲春)有限公司 

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《大鼠补充磷虾油增强认知功能和抗抑郁作用》

Karin Wibrand,Kjetil Berge et al. Lipids in Health and Disease 2013, 12:6


摘要:
背景:
本研究旨在评价磷虾油(KO)对大鼠认知和抑郁样行为的影响。
方法:
采用厌恶性光刺激回避测试(ALSAT)评价认知能力。采用不可避免的厌恶性光刺激(UALST)和强迫游泳试验(FST)评价磷虾油的抗抑郁的作用。丙咪嗪(IMIP)为抗抑郁剂对照品。
结果:
口服磷虾油7周后,雄性和雌性受试大鼠在ALSAT中的活性和非活性之间的识别效果均明显优于对照组(P<0.01)。磷虾油和IMIP都在UALST的第三天阻止了抑郁。同样,与FST对照组相比,KO和IMIP组的固定时间更短(p<0.001)。这些数据支持一种强大的抗抑
郁药,比如ko的潜在和有益的认知效应。研究了前额叶皮质和海马突触可塑性相关基因的表达变化。接受KO补充7周的雌性大鼠海马中,脑源性神经营养因子(Bdnf)的mRNA特别上调(p=0.04),在雄性大鼠中观察到类似的趋势(p=0.08)。雄性大鼠前额叶皮质ARC mRNA的表达也增加,ARC是长期突触可塑性的关键蛋白(p=0.05)。IMIP对包括Bdnf和Arc在内的几个可塑性相关基因有明显的影响。
结论
磷虾油中的活性成分(二十碳五烯酸、二十二碳六烯酸和虾青素)促进了学习过程,并提供了类似抗抑郁的作用。研究结果也表明,磷虾油可能通过与IMIP不同的生理机制发挥作用。
 
Abstract:
Background:
The purpose of the study was to evaluate the effects of krill oil (KO) on cognition and depression-like behaviour in rats.
Methods:
Cognition was assessed using the Aversive Light Stimulus Avoidance Test (ALSAT). The Unavoidable Aversive Light Stimulus (UALST) and the Forced Swimming Test (FST) were used to evaluate the antidepressant-like effects of KO. Imipramine (IMIP) was used as the antidepressant reference substance.
Results: 
After 7 weeks of KO intake, both males and females treated with KO were significantly better in discriminating between the active and the inactive levers in the ALSAT from day 1 of training (p<0.01). Both KO and IMIP prevented resignation/depression on the third day in the UALST. Similarly, a shorter immobility time was observed for the KO and IMIP groups compared to the control in the FST (p<0.001). These data support a robust antidepressant-like potential and beneficial cognitive effect of KO. Changes in expression of synaptic plasticity-related genes in the prefrontal cortex and hippocampus were also investigated. mRNA for brain-derived neurotrophic factor (Bdnf) was specifically upregulated in the hippocampus of female rats receiving 7 weeks of KO supplementation (p=0.04) and a similar trend was observed in males (p=0.08). Males also exhibited an increase in prefrontal cortex expression of Arc mRNA, a key protein in long-term synaptic plasticity (p=0.05). IMIP induced clear effects on several plasticity related genes including Bdnf and Arc.
Conclusions:
These results indicate that active components (eicosapentaenoic acid, docosahexaenoic acid and astaxanthin) in KO facilitate learning processes and provide antidepressant-like effects. Our findings also suggest that KO might work through different physiological mechanisms than IMIP.